中华放射医学与防护杂志

来自 : www.cjrmp.net/zhfsyx/ch/reader 发布时间：2021-03-24

Objective To obtain full-length cDNA of radiation induced new gene RIG1 based on its ESTfragment. Methods Based on non-cloned cDNA library,enhanced nested RACE-PCR and biotin-avidin labeled probe for magnetic bead purification was used to obtain full-length cDNA of RIG1. Results About 1 kb of 3\' end of RIG1 gene was successfully cloned by this set of methods and cloning of RIG1 5\' end is proceeding well. Conclusion The result is consistent with the design of experiment.This set of protocol is useful for cloning of fu11-lengh gene based on ESTfragment.
2001年6月6日目的 在获得了低剂量辐射诱导新基因RIG1 EST片段的基础上,简便、有效地获得其全长cDNA,进行下一步的基因功能研究。方法 结合应用非克隆cDNA文库技术及...中华放射医学与防护杂志